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-50-Phosphopullulan, NEX-MTA cement, G-Bond Plus, MI flow II4045 Pulp Responses to Direct Pulp Capping Material Contains PhosphorylatedPullulanPoster Presentation3:45 PM–5:00 PM Mar 25, 2017CC, First Floor Authors:Authors:ShimpeiKawano (Presenter)Hokkaido University Graduate School of Dental MedicineYu Toida, Hokkaido University Graduate School of Dental MedicineSHIHCHUN TING, Hokkaido University Graduate School of Dental MedicinePipopSaikaew, Hokkaido University Graduate School of Dental MedicineAbu FaemMohammad Chowdhury, Hokkaido University Graduate School of Dental MedicineShinichi Kakuda, Hokkaido University Graduate School of Dental MedicineShigeakiABE, Hokkaido University Graduate School of Dental MedicineShigeakiABE, Hokkaido University Graduate School of Dental MedicineYasushi Shimada, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental UniversityDenis Selimovic, Hokkaido University Graduate School of Dental MedicineYasuhiro Yoshida, Hokkaido University Graduate School of Dental MedicineHidehiko Sano, Hokkaido University Graduate School of Dental MedicineAbstract: Abstract: Objectives: The purpose of this study was to pathologically evaluate the monkey pulpalresponses to a newly developed mineral trioxide aggregate (MTA) direct pulp capping material which contained phosphorylatedpullulanwhich had adhere to the hard tissue and high biological compatibility.Methods: One hundred and twenty cavities were prepared in five monkey teeth. The pulps were intentionally exposed and randomly divided into four groups according to pulp capping materials : a newly developed MTA direct pulp capping material which contained phosphorylatedpullulan(PL, GC), NEX-MTA cement (NX, GC), TheracalLC (TH, Bisco) and Dycal(DY, Dentsply). pullulan(PL, GC), NEX-MTA cement (NX, GC), TheracalLC (TH, Bisco) and Dycal(DY, Dentsply). After that, one-step self-etch adhesive (G bond plus; GC) was applied and filled with flowablecomposite (MI flow 2; GC). The teeth were then extracted after 3, 7 and 70 days, fixed in 10% buffered formalin solution, and prepared according to routine histological techniques to observe pulpalreactions. Tissues were demineralizedand subsequently sectioned. Micrometer sections were stained with hematoxylinand eosin, or alkaline phosphatasefor micromorphologicalobservation.Results: No serious inflammatory reaction of the pulp, such as necrosis or abscess formation, was observed in any of the experimental groups. Disarrangement of odontoblastslayer at 3 and 7 observed in any of the experimental groups. Disarrangement of odontoblastslayer at 3 and 7 days and deposition of thick reparative dentin were the major reaction observed in these materials at 70 days except for DY. For DY, reparative dentin was very thin and dentinal tube was not observed at 70 days. No bacterial penetration along the cavity walls was detected in all groups.Conclusions: Pulp capping with MTA (PL, NX and TH) provided acceptable pulpalresponses and biological compatibility to the monkey pulp, comparable with that of calcium hydroxide (DY). Although PL was promising as direct pulp capping agents, further investigations are required to reveal their cytotoxicityand ability to bond to dentin.reveal their cytotoxicityand ability to bond to dentin.Disclosure Statement:The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONEI have read the IADR policy on licensing.Signed by ShimpeiKawanoReprinted with permission from the Journal of Dental Research, J Dent Res 96 (Spec Iss A):-50-abstract number 4045, https://iadr2017.zerista.com/event/member/332640, 2017