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-45-MI Paste, MI Paste Plus0629 Backscatter Electron Microscopic Imaging of RemineralizationUsing CPP-ACP ProductsPoster Presentation11:00 AM–12:15 PM Mar 23, 2017CC, First FloorAuthors:Authors:Carol Tran, University of QueenslandLei Chai, University of QueenslandHienNgo, University of QueenslandLaurence Walsh (Presenter)University of QueenslandAbstract: Objectives: This small scale in situclinical study assessed a new model for assessing Objectives: This small scale in situclinical study assessed a new model for assessing remineralizationusing white spot lesions (WSL) mounted into orthodontic brackets.Methods: In a double blind randomized crossover trial, enamel slabs with 100 micron deep artificially created white spot lesions were mounted into orthodontic brackets located on the buccalsurfaces of maxillary second premolar and first molar teeth. 4 subjects used in random order MI Paste, MI Paste Plus, a prototype of MI Paste Plus with enhanced mineral release, or a vehicle paste containing 900 ppmfluoride. All products were in coded tubes and had identical flavours. Both treatment and washout periods were 2 weeks. Electron probe microanalysis was undertaken as well as backscatter electron imaging (BSE) of cross-sectional enamel. Image undertaken as well as backscatter electron imaging (BSE) of cross-sectional enamel. Image analysis of grey scale data from BSE was used to compare differences in mineral gain according to changes in mineral density of the subsurface lesionResults: The two products containing both CPP-ACP with fluoride produced identical actions and both gave significantly greater mineral gain in WSL than CPP-ACP alone, which in turn was better than fluoride alone. Alterations in the BSE grey scale levels from the enamel surface through the lesion correlated with changes in calcium and phosphorus levels assessed using EPMA.Conclusions: This small scale study is a useful proof of concept for this clinical model where exposure of WSL to saliva and toothbrushingis enhanced. The clinical factors in this model exposure of WSL to saliva and toothbrushingis enhanced. The clinical factors in this model include high access to parotid saliva, regular brushing of enamel surfaces and twice daily topical use of products by highly compliant healthy subjects. The study also shows how BSE can be used to rate the effect of various agents on remineralization. Acknowledgements: This work was supported by the CRC Oral Health and the Australian Dental Research Foundation.Reprinted with permission from the Journal of Dental Research, J Dent Res 96 (Spec Iss A):-45-abstract number 0629, https://iadr2017.zerista.com/poster/member/75415, 2017